Abstract
Introduction: Ability to coat prosthetic grafts with endothelial cells has been practised for a long time [1] and recent reviews [2] have favoured a two stage approach. The Achilles heel of these technologies has been finding a suitable matrix which enables the endothelial coat to adhere to theprosthesis.Herewewould like topresent our results with an innovative matrix using cultured human endothelial cells. Methods: Endothelial cells were obtained from human saphenous veins and internal thoracic arteries after collagenese treatment and were expanded million folds in vitro.Aftermodifying surfacepropertiesof ePTFE tubings, we optimised conditions that would enhance adhesion of these endotheial cells. Adhesion assays were carried out using 96 well ELISA plates. We treated the hydrophobic plastic surfaces with ethanol / methanol and an acidified
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