Abstract
This method is based on the theory that positive polymer ions combine stoichiometrically with negative ones in a dilute solution. The solution of a positive polyelectrolyte like N-trimethylated glycol chitosan is added to a solution or a plant extract which contains pectic acid derived from pectin by demethylation of the latter with alkali, the excess of the added positive polyelectrolyte being titrated with the standard solution of a negative polyelectrolyte like potassium salt of polyvinyl sulfonate. The end-point of this titration is indicated by the color change of toluidine blue or sudden precipitation of the reactants. The amounts of pectin, expressed as pectic acid, are calculated from the difference between the results of the titration and the blank experiment. This method is also applicable to estimation of the methoxyl contents of pectin.
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