A new method for the preparation of three-layer vascular stents: a preliminary study on the preparation of biomimetic three-layer vascular stents using a three-stage electrospun membrane

Abstract

There is an urgent need to design a tissue-engineered vascular graft that exhibits good biocompatibility and sufficient mechanical strength to repair and facilitate regeneration of defective vascular tissue. It is generally accepted that multi-layer stents can be used to simulate the structure and function of natural blood vessels. Here, we developed a new three-layer tubular graft that is rolled from a single Poly(L-lactide-co-caprolactone) electrospun membrane. We used a new electrospinning technique to place three different structures on a single electrospun membrane such that the stent is comprised of three different layers. The inner layer is dense and suitable for endothelial cell growth, the middle layer is a parallel loose structure suitable for smooth muscle cell growth, and the outer layer is a parallel structure with sparse alternating texture suitable for both smooth muscle cell growth and structural support. The vascular stent has good tensile strength. At the same time, endothelial cells and smooth muscle cells readily proliferate on the material in vitro. In particular, smooth muscle cells grow in parallel on the middle and outer materials. In vivo, all layers of the vascular graft were infiltrated by cells within one week of subcutaneous implantation, indicative of favorable biocompatibility. After a week of subcutaneous implantation, the vascular stent was orthotopically transplanted into the abdominal aorta of Sprague Dawley rats. After ten weeks of transplantation, ultrasound imaging of the abdomen showed vascular patency. The vascular stent was endothelialized, smooth muscle cells readily proliferated, and a large amount of elastic fibers were formed. Therefore, our specially designed tri-layer vascular graft may be of significant benefit in vascular reconstruction.