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Abstract
A new method for the determination of trapped plasma in packed red cells, using Indocyanine green as a marker, is described. The method was applied to measure sodium and potassium content in human red blood cells. The means of the three parameters studied, calculated from duplicate determinations on 39 normal subjects, are included in the following ranges with a confidence coefficient of 95%: Trapped plasma % = 7.10 ± 2.47; Na + mequiv/10 13 cells = 7.45 ± 3.72; K + mequiv/10 13 cells = 91.15 ± 22.41. Statistical evaluation of the data confirmed the reliability of the method. The results were reproducible and agree well with those obtained by other authors who used radioactive markers. Furthermore the new technique is cheap and requires a short time to set up.
Published Version
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