Abstract
Phellodendri Amurensis Cortex (PAC) is a well-known herbal medicine in China with complex components, but the previous research has mostly focused on its alkaloids analysis. For the first time, a simpler and more efficient method was proposed in this paper to simultaneously determine the content of three different kinds of compounds—phenolic acids, alkaloids and limonoids—in PAC. The phenolic acids included 3-O-feruloylquinic acid, 4-O-feruloylquinic acid and syringin. The alkaloids include magnoflorine, phellodendrine, jatrorrhizine, palmatine and berberine, while the limonoids include obaculactone and obacunone. An approach combining multi-wavelength and HPLC-DAD was used in this study due to the great difference in maximum absorption wavelength of the various components. Four wavelengths at 215, 275, 280 and 310 nm, respectively, were chosen for monitoring. It has been indicated through appropriate tests that this approach is of high accuracy, good repeatability and stability and provides a scientific basis for the quality assessment of PAC and associated derivatives. In addition, the chromatographic fingerprints method combined with multivariate statistical analysis chosen in this study was proved to be effective and reasonable for an accurate classification of 33 batches of samples collected from different locations.
Highlights
Phellodendri Amurensis Cortex (PAC), known as “Guan Huang Bai” in China, is derived from the dried bark of Phellodendron amurense Rupr. and mainly distributed in the northeastern region of China [1]
In addition to the traditional efficacy of clearing heat and eliminating dampness, detoxifying and eliminating inflammation; further biological activity has been discovered in modern pharmacological studies of PAC, including antioxidant, hypoglycemic, antitumor activity, induced specific immune tolerance, neuroprotection and so on [2,3,4,5,6,7]
Phytochemical studies in the literature show that PAC was known to contain a complex mixture of ingredients, including alkaloids, phenolics, limonoids and so on [12,13,14]
Summary
Phellodendri Amurensis Cortex (PAC), known as “Guan Huang Bai” in China, is derived from the dried bark of Phellodendron amurense Rupr. and mainly distributed in the northeastern region of China [1]. Phytochemical studies in the literature show that PAC was known to contain a complex mixture of ingredients, including alkaloids, phenolics, limonoids and so on [12,13,14]. Most studies have on the biological activities of the alkaloids derived from PAC, including their antimicrobial activity [15], reported on the biological activities of the alkaloids derived from PAC, including their antimicrobial anti-inflammation activity [16], and neuroprotective activity [5,17]. Phenolic acids are a widely activity [15], anti-inflammation activity [16], and neuroprotective activity [5,17]. Phenolic acids are distributed group of secondary metabolites that possess strong anti-oxidative activities, and they a widely distributed group of secondary metabolites that possess strong anti-oxidative activities, areand generally into added medicinew to preventto cancer andcancer bacterial growth they areadded generally into medicinew prevent and cell bacterial cell[18,19]
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