Abstract
Bleomycin (BLM) cytotoxicity was greatly enhanced by vortex-stirring BLM and mammalian cells for a few seconds together with 1 to 10 microg/ml high molecular weight polyacrylic acid (A-119). When suspensions of murine leukemia P388 cells were injected together with BLM and A-119 into the tail vein of CDF1 mice, cell viability was reduced to 1/1000, the reduction being similar to that obtained by vortex-stirring the cells with BLM and A-119 in vitro. This was corroborated by an increase in the survival time of these mice. The reduction in cell viability was noted only when the cells, BLM, and A-119 were simultaneously injected. There was absolutely no effect when there was a time-lag between cell inoculation and injection of BLM/A-119. These findings suggest that the conditions created by the blood stream may simulate those of vortex-stirring and that, in both cases, rapid uni-directional movement of cells with high molecular weight polyacrylic acid may affect the plasma membrane facilitating internalization of non-permeant materials into cells.
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