A New Method for Hybrid Bermuda Grass (Cynodon dactylon × C. transvaalensis Burtt.-Davy) Vegetative Propagation

Abstract

Hybrid Bermuda grasses (Cynodon dactylon × C. transvaalensis Burtt.-Davy) represent one of the greatest contributions to the growing quality of turfgrass in the warm season and transition zone areas of the world. Hybrid Bermuda grass production relies on vegetative propagation from sod or sprigs. In the past, efforts have focused on improving the technique of stolonizing (or sprigging) for establishment in new areas. Such propagation requires bulk harvesting and planting of all rhizomes and stolons. We have developed a novel method of propagation and establishment from a single node harvested from greenhouse grown stolons. Despite a stolon fraction bearing a single node being suitable for effectively propagating a warm-season turfgrass, the technique has been held as economically impractical until now. Our method has been developed to obtain the multiplication of plant material in soilless conditions by harvesting single-node sprigs, propagation of plants from the single nodes, and transplant of single plants in the field. The investigation aimed to identify values for method set-up. Indeed, node and internode size variability with differential between maximum diameters is crucial for discrimination. For Patriot Bermuda grass stolons, nodes exhibited a maximum diameter of 2.43 ± 0.46 mm, while internodes had a maximum diameter of 1.54 ± 0.16 mm. Based on these findings, a 2 mm sieve was selected, achieving an optimal ratio between the node fraction and internode residues. The sieve yielded 87% of node fractions and only 1% of internodes from the initial mix, demonstrating its efficacy. Further results for the transplanting phase indicated that a double release resulted in an average success rate of 98.8%, with only 6.9% blank cells when using a single release. The average was 149 plants per tray over 160 cells, representing a 93.1% success rate. These results underscore the efficiency and acceptability of the overall propagation process in alignment with market references.