Abstract
Rat pancreas, embedded in 2-hydroxyethyl methacrylate (GMA), was used to demonstrate glucagon by immunofluorescence in combination with a hematoxylin/eosin-phloxin (H/E-P) stain. This method for GMA embedded tissue enables a good differentiation with both light and fluorescence microscopy. The enzyme-labeled-fluorescence (ELF) precipitate marked immunoreaction can be visualized with UV excitation, whereas the H/E-P stain can be observed in both bright field and with blue or green excitation. The ELFsignal together with the surrounding tissue structure can be visualized with double bandpass filters that enable UV together with blue or green excitation. Alternatively a similar effect can be achieved photographically by double exposure. (The J Histotechnol 21:25, 1998)
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