A new method for electrochemical determination of Hippuric acid based on molecularly imprinted copolymer

Abstract

This study introduces easy detection of Hippuric Acid (HA) through electrochemical polymerization technique. The molecularly imprinted polymer (MIP) was immobilized on a glassy carbon electrode (GCE) through electropolymerization of monomers, including m-dihydroxy benzene (m-DB) and o-aminophenol (o-AP), in presence of HA as the template. Moreover, it was subsequently delineated using electrochemical probe of hexacyanoferrate redox, atomic force microscopy (AFM), fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and electrochemical techniques such as cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). This sensor exhibited limit of detection (0.012 nM) with a good linear range of 0.05–40 nM and 40–500 nM. The developed sensor was properly employed to determine HA in real samples with acceptable results.