Abstract
Cells are cloned on small (150 × 300 μ) ‘glass platelets’, suspended in agarose, above a layer of feeder cells, likewise suspended in agarose. At confluence, individual platelets are lifted into 16 mm tissue culture wells; cell sheets grow out from the platelets onto the wells, and at confluence are passaged in the usual way. Overall cloning efficiency — from single cell on platelet, to confluent 9 cm dish — was high: 47% for secondary cells (whole mouse embryo), and 72% for an established line (3T3). The method seems to be suited to the study of interactions via diffusible exudates, between different combinations of anchored and suspended cells.
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