Abstract
Abstract—A new strain of methanogenic archaea, designated VTT, was isolated from a sample of Spitsbergen permafrost. The cells were nonmotile curved rods, 2.7–5.3 × 0.3 µm. The optimal conditions for growth were 20°C, pH 6.6, and NaCl concentrations 0.03–0.05 M. The H2/CO2 gas mixture was the only substrate used. In the presence of H2/CO2, growth was stimulated by addition of yeast extract or rumen fluid. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain VTT belonged to the genus Methanobacterium and was most closely related to M. lacus 17A1T (97.02% similarity). Comparison of the sequenced and assembled genome of strain VTT with the genomes of other members of this genus confirmed these results and revealed species-level differences. Our results indicate that this methanogenic isolate belongs to a new species of methanogenic archaea, for which the name Methanobacterium spitsbergensе sp. nov. was proposed, with the type strain VTT (=VKM B-3566T = JCM 39284T).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
