Abstract
This study investigates the role of the soft tissue bed in rhBMP‐2‐induced bone formation in large segmental bone defects. We hypothesized that delivery of rhBMP2 triggers endogenous BMP2 release by the soft tissue bed of the defect. Thirty‐five millimeter defects were created in the mandibles of five foxhound male dogs and immediately reconstructed with 8ccs of rhBMP‐2 (0.2 mg/ml) infused into absorbable collagen sponge (ACS). Soft tissue samples were collected 12 weeks after reconstruction. Gene expressions of endogenous BMP2 as well as 28 related genes were measured in the collected samples using real‐time PCR (qrt‐PCR) arrays. Analysis of the mRNA at treated sites revealed significant up‐regulation in the expressions of several genes related to BMP2 and inflammatory pathways. The highest significant up‐regulation detected in COL1A1 (+10.6 folds), BMP2 (+9.5 folds). Protein extracted from the periosteal tissue at the regenerate sides showed statistically significant higher concentration of BMP2 than contralateral sides. These results suggest that rhBMP‐2 grafting stimulates the production of endogenous BMP‐2 in the tissue pad surrounding the defect.This study was supported in part by Medtronic, inc.
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