A new LC\u2013MS/MS method for multiple residues/contaminants in bovine meat

Zehra Hajrulai-Musliu,Lazo Pendovski,James Jacob Sasanya,Stefan Jovanov,Velimir Stojkovski,Dean Jankuloski,Risto Uzunov

doi:10.1186/s13065-021-00788-5

Zehra Hajrulai-Musliu, Lazo Pendovski + Show 5 more

Open Access

https://doi.org/10.1186/s13065-021-00788-5

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Abstract

A multi-class and multi-residue/contaminant method for the determination of veterinary drug and pesticide residues and mycotoxins in bovine meat has been developed and validated. The veterinary drug residues/contaminants included antimicrobials, anabolic hormones, lactones, β-agonists, mycotoxins, and pesticides. Isotopic labeled internal standards were included to compensate residual matrix effects. The calibrators used in the method demonstrated linearity with the R2 > 0.98. The decision limit (CCα) values were in the range from 0.067 to 2103.84 μg/kg, while the range for detection capability (CCβ) was from 0.083 to 2482.13 μg/kg. The limit of detection (LOD) and limit of quantification (LOQ) were in the range from 0.059 to 291.36 μg/kg, and 0.081 to 328.13 μg/kg, respectively. The recovery of analytes ranged from 61.28% to 116.20%. The intra-day coefficient of variation (CV) was from 0.97 to 25.93% and the inter-day CV was 2.30–34.04%. The method has been used for the determination of 49 residues/contaminants in bovine meat. Application of the method in routine analysis in bovine samples, revealed in limited samples the presences of enrofloxacin, oxytetracycline and sulfadiazine at the concentration of 35.22 µg/kg, 27.35 µg/kg, and 36.20 µg/kg, respectively.

Highlights

Important source of nutrition for many people around the world is meat
Liquid chromatography tandem mass spectrometry (LC–MS/MS) techniques provide a universal approach applicable to the widest number of veterinary drug residues and it is today become the technique for analysis of drug residues and contaminants in food stuffs
The present paper describes a sensitive and reliable LC–MS/ MS including simple and generic method for the analysis of 49 veterinary drug residues and other contaminants in bovine meat

Summary

Introduction

Important source of nutrition for many people around the world is meat. The safety of meat is seriously challenged by all kinds of low molecular weight organic contaminants, such as residues of veterinary drugs, agro-chemical residues, mycotoxins, food additives and environmental contaminants, which arouse considerable attention from people over the world [12, 14]. The development of simultaneous multi-class drug residue determination is challenging task because concentrations of analyte are low in the tissues such as meat. The mass spectrometry is selective detection technique, the sample preparation step in analytical procedures is necessary because that reduces interference and matrix effect which occurs with the use of mass spectrometry, especially when using electrospray ionisation (ESI). Techniques such as immunoaffinity chromatography, liquid–liquid extraction, solid phase extraction (SPE) and matrix solid-phase dispersion (MSPD) are used for extraction and purification.

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