Abstract
A New LC/MS/MS Method for the Analysis of Phyllanthin in Rat Plasma and its Application on Comparative Bioavailability of Phyllanthin in Different Formulations after Oral Administration in Rats
Highlights
A simple, short ultra performance liquid chromatography (UPLC)/MS/MS method for quantitation of phyllanthin in rat plasma in less than 2 minutes have been developed and fully validated
We evaluate pharmacokinetic features of phospholipid complex of standardized P. amarus extract (PAE) compare with the standardized P. amarus extract on rats after oral administration
Selectivity: The selectivity was evaluated by analyzing rat blank plasma, blank plasma spiked with phyllanthin, and a rat plasma sample after dosing
Summary
A simple, short UPLC/MS/MS method for quantitation of phyllanthin in rat plasma in less than 2 minutes have been developed and fully validated. The validated method was used to investigate the pharmacokinetic properties of phyllanthin in PA extract and phospholipid complex of PA extract in rat. Phyllanthin is extracted from a small volume of rat plasma (100 μl) by means of liquid-liquid extraction method with tert butyl methyl ether. Conclusion: A simple, short UPLC/MS/MS method for quantitation of phyllanthin in rat plasma in less than 2 minutes have been developed and fully validated. The bioavailability of phyllanthin from the phospholipid complex of PA extract in rat plasma was significantly improved compared with that of raw PA extract after oral administration. There are several methods for the quatitative analysis of phyllanthin from rat plasma such as HPLC–fluorescence detection[10,12], HPLC with PDA detection method[11,12], and these methods
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