Abstract
A comprehensive research has been carried out on l-glutaminase producers from marine bacteria. Among the evaluated isolates, Kosakonia radicincitans was found to be a l-glutaminase producer from a marine environment, having the highest enzymatic activities. The optimal condition for l-glutaminase production from K. radicincitans was at pH and temperature of 7.0 and 37 °C, respectively. The enzyme was found stable between pH 4 and 8 and its function also maintained at temperatures between 31 and 43 °C after 30-min incubation. Both the amino acid sequence and domain analysis of the l-glutaminase from the Kosakonia strains revealed various similarities and dissimilarities between these amidase enzymes. Sequence analysis and comparison of l-glutaminase from Kosakonia sp. with other l-glutaminase along with structural prediction were able to identify the unique characteristics of the predicted structures and offer a different perspective into their distinguishable properties for industrial use.
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