Abstract
BackgroundPrevious studies have shown that changes in intestinal microfloras are associated with both gastrointestinal (GI) and non-GI tumors. It is not clear whether there is an association between GI microflora changes and hematological malignancies.MethodsIn the current study, we used 16S rDNA gene sequencing techniques to profile the GI microbiome in children with lymphoblastic leukemia (ALL, n = 18) and matched healthy control (n = 18). Using multiple specialized software [Heatmap, Principal coordinates analysis (PCoA), Claster and Metastates], we analyzed the sequencing data for microfloral species classification, abundance and diversity.ResultsA total of 27 genera between the ALL and control groups (FDR ≤ 0.05 and/or P ≤ 0.05) showed significantly different abundance between ALL patients and healthy controls: 12 of them were predominant in healthy group and other 15 species were significantly higher in ALL group. In addition, we compared the abundance and diversity of microfloral species in ALL patients prior to and during remission stage after chemotherapy, and no significant difference was detected.ConclusionsCompared to healthy controls, ALL patient showed significant changes of GI microfloras. Further explorations of the intestinal micro-ecology in ALL patients may provide important information to understand relationship between microfloras and ALL.
Highlights
Previous studies have shown that changes in intestinal microfloras are associated with both gastrointestinal (GI) and non-GI tumors
We explored the intestinal microfloras in children with Acute lymphoblastic leukemia (ALL) in comparison to age- and sex-matched healthy controls using the 16S rDNA high-throughput sequencing technique
Our findings from the current study provided new insight on ALL pathogenesis, which either results in the changes of intestinal microfloras or its development/progression clarity an involvement of the change of microbiome
Summary
Previous studies have shown that changes in intestinal microfloras are associated with both gastrointestinal (GI) and non-GI tumors. It is not clear whether there is an association between GI microflora changes and hematological malignancies. Previous studies have shown that changes in intestinal microfloras or their dysfunction were closely associated with human health and the development and progression of diseases [7, 8]. Our findings from the current study provided new insight on ALL pathogenesis, which either results in the changes of intestinal microfloras or its development/progression clarity an involvement of the change of microbiome
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