Abstract
Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.
Highlights
Maslinic acid [MA, (2α,3β)-2,3-dihydroxylolean-12-en-28-oic acid] and oleanolic acid (OA, 3β-hydroxylolean-12-en-28-oic acid) are two main pentacyclic triterpenic carboxylic acids found in the olive tree (Olea europaea) [1]
The effects that the MA concentration in the cell culture exerts on growth survival has been well characterized and the concentration required for 50% growth inhibition (IC50) has been well determined for different types of cells [16,22]
We examined the effect of the mixture of MA and OA (98:2, w:w) on the proliferation of HT29 and
Summary
Maslinic acid [MA, (2α,3β)-2,3-dihydroxylolean-12-en-28-oic acid] and oleanolic acid (OA, 3β-hydroxylolean-12-en-28-oic acid) are two main pentacyclic triterpenic carboxylic acids found in the olive tree (Olea europaea) [1]. MA has been found to exert important effects such as growth stimulating [2,3,4,5,6] anti-oxidant [7,8,9,10,11], anti-inflammatory [12], anti-microbial and anti-viral [13,14,15], and even anti-tumoral [16,17,18,19,20] With respect to the latter, it is of great interest that MA selectively inhibits cell proliferation in human colon-cancer HT29 cell line [17]. The methods used in different plant samples or other biological samples have been based on HPLC-UV/vis or gas chromatography (GC)-MS [1,23,24,25] With these procedures the small amounts of MA present in cultured cells cannot be precisely determined. They constitute a good tool to investigate the cytoprotective, genotoxic, and antigenotoxic effects of compounds, to understand hepatocarcinogenesis, and to study drug targeting [27,28]
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