A new hemorheological approach for evaluation of antiplatelet and anticoagulative drugs – Rotational viscometry

Abstract

Based on rotational viscometry of clotting, an approach for evaluation of antiplatelet drugs and anticoagulants was explored. Rat platelet rich plasma (PRP) and plate­ let poor plasma (PPP) were prepared. PRP and PPP were seperately incubated with and without drug (D), namely, PRP, PRP+D, PPP, and PPP+D. Recalcification of the sam­ ples were monitored by HAAKE Rotovisco RV20/CV100 at 0.3 s-l The inhibition of platelet involvement (IPI) and the inhibition of plasma clotting(IPC) were defined. According to three clotting parameters (tr, d~/dt, ~m), six inhibitions (3 IPI, 3 IPC) were obtained for evalu­ ating a drug. The preliminary investigation on heparin, pentoxifylline, aspirin, notoginseng saponins and alco­ hol indicated that the approach would be a promising mean for pharmacological and clinical practice.