Abstract
As one of the essential trace elements in metabolism, iodine is crucial to maintain the normal physiological functions. Therefore, based on health and environmental protection, it is very important to realize sensitive detection of iodide ion. Herein, we developed a simple, rapid and sensitive method for the determination of iodide ion. Trypsin was used as an ideal template for the synthesis of gold nanoflower sol (AuNFs) with anisotropic surface structure and good stability. It exhibits highly active surface enhanced Raman scattering (SERS) effect and can be used as facile SERS sol substrate. The TMBox generated by the catalytic oxidation reaction of TMB-chloramine T-iodide ion is used as the SERS probe. The enhanced SERS signal intensity is linearly related to the iodide ion with high sensitivity. In addition, TMB has fluorescence effect, and the colored TMBox can produce RRS signal due to polymerization. Based on this, a quad-mode detection method of SERS, RRS, fluorescence and colorimetry for quantitative detection of trace iodide ions was established, and this method can be applied to the detection of iodide ions in natural water and drinking water.
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