Abstract
A novel water-soluble polysaccharide, designated Bletilla striata polysaccharide b (BSPb), was isolated from a traditional Chinese medicinal herb, Bletilla striata. The crude polysaccharide was obtained by hot water extraction and purified by ion-exchange chromatography. The molecular weight was 260kDa as determined by HPGPC (high performance gel permeation chromatography). Monosaccharide analysis showed that BSPb contained glucose and mannose with a molar ratio of 3:1. Methylation and spectroscopic analyses with 1D and 2D NMR spectroscopy showed that the backbone of BSPb is mainly composed of (1→2)-linked α-d-mannopyranose and (1→4)-linked β-d-glucopyranose residues. In this study, BSPb plays an important role in protection against the renal fibrosis effect, which is probably mediated by down-regulated TGF-β RI, TGF-β RII, and α-SMA in vitro.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
