Abstract

A reliable, fast, and low‐cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9‐di (6,6′‐ N, N′ trimethylammonium) hexylfluorenyl‐2, 7‐diyl)‐alt‐co‐ (1,4‐phenylene)] dibromide salt (PFP) has been taken as cationic conjugated polymer (CCP) and PNA attached with fluorescein dye (PNAC∗) as a probe. The basic principle of this sensor is that when a PNAC∗ probe is hybridized with a single strand DNA (ssDNA) having complementary sequence, Forster resonance energy transfer (FRET) may take place from PFP to the PNAC∗/DNA complex. If the FRET is efficient, the photoluminescence from the PFP will be highly quenched and that from PNAC∗ will be enhanced. On the other hand, if the DNA sequence is noncomplementary to PNA, FRET will not occur.

Highlights

  • Conventional methods of medical diagnostics are generally very expensive and time consuming and are mostly based on antibody detection of the infected person

  • We report the method of preparation, operation, and application of a biosensor that provides fast, sensitive, and selective detection of DNA hybridization by using water-soluble cationic conjugated polymer (CCP) and PNA probe

  • The condition of maximum overlap between the emission spectrum of the donor PFP and the absorption spectrum of the acceptor PNAC∗ for efficient Forster resonance energy transfer (FRET) to occur is satisfied as is clear from Figure 2

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Summary

INTRODUCTION

Conventional methods of medical diagnostics are generally very expensive and time consuming and are mostly based on antibody detection of the infected person. Its application areas include clinical diagnostics, single nucleotide polymorphism (SNP) genotyping, environmental studies, antiterrorism, and forensic analysis [1] This has motivated the development of novel DNA sensors with optical [2,3,4,5,6], acoustic [7, 8], or electronic [9,10,11,12,13] readout. CPs may be viewed as a collection of short, conjugated (oligomeric) units kept in close proximity by virtue of the polymer backbone Their structure allows effective electronic coupling and fast intra- and interchain energy transfer [20, 21]. FRET between the donor polymer and the acceptor PNAC∗ is used to detect the Journal of Sensors

B Anneal
Materials
Spectroscopic measurements
RESULTS AND DISCUSSION
CONCLUSIONS
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