Abstract
This paper reports on the synthesis and characterization of a new selective fluorescence probe for gallium ions in water and living cells. Hydrazone of pyridoxal 5′-phosphate and thiophene-3-carbohydrazide was demonstrated to be a “turn-on” fluorescence sensor that can selectively detect Ga3+ with a noticeable appearance of cyan fluorescence in aqueous DMSO. The complex stoichiometry of the probe for Ga3+ was determined through various methods and its structure and electronic spectra were optimized by quantum chemical calculations. The probe showed a low detection limit of 34 nM, and is suitable for river water analysis with high ion recovery and exhibits low toxicity on different cell lines. This study provides valuable insights into the design and optimization of fluorescence probes for selective detection of metal ions.
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