Abstract
A new pH-sensitive fluorescent probe NAP-MDA was designed and synthesized. NAP-MDA consists of 1,8-naphthalimide as fluorophore, morpholine and N,N-dimethylethylenediamine as pH-responsive groups. Due to the photoinduced electron transfer (PET) mechanism, the fluorescence of 1, 8-naphthalimide was thoroughly quenched under alkaline condition (pH > 10.0), however, NAP-MDA displayed increasing fluorescence as the rise of acidity. Notably, NAP-MDA possessed an excellent linear dependence with neutral to alkaline pH (7.2–9.4), with a pKa of 8.38. NAP-MDA had good photostability and reversibility. Meanwhile, the probe was selective to pH without interference from common reactive species, temperature and viscosity. Fluorescent testing strips were fabricated with NAP-MDA and were successfully utilized to visualize the different pH with a handhold UV lamp. Confocal fluorescence imaging in live cells demonstrated that NAP-MDA mainly fluoresced in lysosomes, and could be applied for quantification of the pH within live cells.
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