Abstract
Effective DNA purification is essential for reliable composition and contamination analysis in the food industry. This paper compares two DNA extraction and purification procedures for food analysis. The commercially available DNA extraction kit from Agilent Technologies, Inc. was tested against a new solid phase extraction (SPE) method using silica beads embedded in a porous monolith structure that is integrated in a disposable column design. The technique is amenable to automation and streamlined workflow integration, with possible miniaturization of the sample handling process making it suitable for high-throughput applications. To demonstrate the automated process, the manual homogenizing step was compared to a custom automated procedure. To demonstrate the process for food testing, two types of sausages were analyzed for traces of porcine gDNA.
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