Abstract
The design and implementation of a computer-controlled pH-stat is presented. The reagent delivery system used in the design of the pH-stat is based on a computer-controlled droplet generator able to add selectively reagent aliquots of 0.06 μl. Precise reagent control enables the stationary control of pH to within 0.0008 pH units. Algorithms for program operation are structured as autonomous concurrent tasks and are written entirely in FORTRAN. The pH-stat is operated by means of simple directives entered into a terminal. During an experiment, directives permit the graphic display of data and the alteration of experimental parameters. To evaluate the pH-stat. standard working curves were obtained from which the concentration of glucose in a sample of standard human blood serum was determined to within 3% of the value specified by the manufacturer. Two alternative measurement approaches, the fixed-time technique and the use of enzymatic induction times, were evaluated for the determination of glucose concentrations.
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