A New Direction for Conifer Genomics

Abstract

Extensive genome resources have now been developed for both spruce and pine. However as other technologies develop and costs drop, other resources become feasible. One such resource, not yet available in conifers, is collecting complete cDNA sequences (full length messenger RNA transcripts). We describe our approach for obtaining such sequences and the importance of this resource. Second, existing resources and new technologies are allowing new approaches in functional and comparative genomics. In functional genomics, new technologies and the integration of mRNA, protein and metabolite expression profiling promises to improve the identification of gene function, interaction and regulation. In comparative genomics, an apparent slowdown of evolutionary rate in the pine family should allow genome information to be easily transferred between spruce and pine (and other members of the pine family). Ultimately, genomics research should impact practical problems and traits in breeding and management programs. In our own program, we target spruce resistance mechanisms to the spruce weevil, a serious pest in British Columbia.