Abstract
The intrauterine inseminator (IUI) was developed to provide the method of depositing dog semen into the uterine body instead of the vagina. The IUI consists of a vaginal endoscope, a balloon sheath, and injection catheter. When the endoscope is inserted into the vagina and the balloon expanded with air, the cervical os becomes visible so a injection catheter can be inserted through the cervix for deposition of the frozen-thawed semen. The efficacy of the IUI device was compared to intra-vaginal artificial insemination using semen that had been collected and frozen from pooled sperm-rich fraction of ejaculates collected from two Jindo dog donors. Aliquots of semen were extended with a Tris-egg yolk diluent, centrifuged, the seminal plasma removed, the pellet resuspended with the same diluent, and cooled to 5°C over a 2 h period. A Tris-egg yolk-glycerol extender was added at 5°C; after 1 h, semen was loaded into 0.5 ml straws, and straws were frozen in LN vapor for 5 min, and immersed in LN for storage. The final sperm concentration for freezing was approximately 100×10 6 cells/ml. The straws were thawed at 70°C for precisely 6 sec, 1.5 ml Tris-egg yolk buffer at 38°C added, and the 2 ml of thawed semen was used for a single insemination using the IUI device. Each bitch was inseminated at optimal insemination point, which was estimated by vaginal epithelial cells staining and progesterone concentration analysis. Use of the IUI device resulted in 21 of 26 females giving birth to 89 pups (4.2±1.6 pups per litter), while intra-vaginal AI resulted in 6 of 15 females whelping a total of 17 pups (2.8±1.2 pups per litter). We believe the IUI device is easier to use than previously described devices used for intrauterine insemination. In our experience the expansion of the balloon has a calming effect on the bitch that aids the inseminator. These results indicate that the IUI device was able to provide high fertility with 50 million frozen sperm per insemination and two inseminations. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 2 : 180-184)
Highlights
Several factors are critical to obtain a high fertility rate with artificial insemination (AI) of frozen canine spermatozoa, e.g., high initial semen quality, proper timing of the insemination (Farstad and Andersen Berg, 1989; Linde-Forsberg and Forsberg, 1989; Linde-Forsberg and Forsberg, 1993) and intrauterine semen deposition (Fontbonne and Badinand, 1993; Govette et al, 1996).Semen quality after freezing and thawing and the number of spermatozoa required per insemination will, greatly depend on the quality of ejaculated semen, how well it survives the freezing and thawing process, and techniques used for the freezing-thawing process
These results indicate that the intrauterine inseminator (IUI) device was able to provide high fertility with 50 million frozen sperm per insemination and two inseminations
For insemination the endoscope is inserted into the vagina and the balloon sheath expanded with air using a Figure 2
Summary
ABSTRACT : The intrauterine inseminator (IUI) was developed to provide the method of depositing dog semen into the uterine body instead of the vagina. The efficacy of the IUI device was compared to intra-vaginal artificial insemination using semen that had been collected and frozen from pooled sperm-rich fraction of ejaculates collected from two Jindo dog donors. The straws were thawed at 70°C for precisely 6 sec, 1.5 ml Tris-egg yolk buffer at 38°C added, and the 2 ml of thawed semen was used for a single insemination using the IUI device. In our experience the expansion of the balloon has a calming effect on the bitch that aids the inseminator These results indicate that the IUI device was able to provide high fertility with 50 million frozen sperm per insemination and two inseminations.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
