Abstract
Several coupling factors have been isolated from mitochondria ( Racker, 1967) which are required for oxidative phosphorylation in resolved submitochondrial particles. Phosphorylation in chloroplasts, like that in mitochondria, can be resolved and reconstituted ( Avron, 1963; Vambutas and Racker, 1965), however, only one coupling factor, CF 1 1 1 Abbreviations: CF 1, CF 2 — chloroplast coupling factor 1 and 2, respectively; F 1, F 4 — mitochondrial coupling factor 1 and 4, respectively; SCP — subchloroplast particles; T-SCP — trypsin-treated subchloroplast particles; Pi — inorganic phosphate; DTT — dithiothreitol; DTT-ATPase — ATPase of CF 1 activated by DTT. which in several respects is similar to mitochondrial F 1 ( McCarty and Racker, 1966) has so far been isolated. The purpose of this communication is to report the discovery of a second coupling factor in a protein fraction obtained by alkaline extraction of spinach chloroplasts. This factor (CF 2) restored photophosphorylation in deficient chloroplast particles in the presence of added CF 1 and also interacted with CF 1 in solution.
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