Abstract
A novel potentiometric, enantioselective membrane electrode based on graphite paste (graphite powder and paraffin oil) has been constructed. The graphite paste is impregnated with 2-hydroxy-3-trimethylamoniopropyl-β-cyclodextrin (as chloride salt) solution, 10−3 mol/L. The potentiometric, enantioselective membrane electrode can be used reliably for L- proline assay with the chronopotentiometry (zero current) technique, in the 5.0 x 10−5 - 1.5 x 10−1 mol/L concentration range (detection limit 1.0 x 10−5 mol/L), with an average recovery of 99.90% (RSD = 0.12%). The response characteristics of the enantioselective, potentiometric membrane electrode were also determined for D-proline. The surface of the electrode can be regenerated by simple polishing, obtaining a fresh surface ready to be used in a new assay.
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