A new configuration of sequencing batch reactor operated as a modified aerobic/extended-idle regime for simultaneously saving reactor volume and enhancing biological phosphorus removal

Abstract

Recently, it has been reported that the aerobic/extended-idle (AEI) regime can achieve a satisfied biological phosphorus removal (BPR). Although the AEI regime has exhibited some merits, its main drawback that the extended-idle phase (e.g., 210–450min) is much longer than the anaerobic phase (e.g., 60–120min) performed in the aerobic/oxic (A/O) regime requires to be addressed. In this study, a new configuration of sequencing batch reactor (SBR) with sludge tank halved (STH-SBR) was therefore designed. After stable operation, 96.9±0.5% of total phosphorus was removed in the STH-SBR, which was higher than that in the AEI-SBR (86.9±0.8%) and A/O-SBR (84.7±1.3%). Further investigations showed that the biomass cultured in the STH-SBR contained more polyphosphate accumulating organisms but less glycogen accumulating organisms than that in the AEI-SBR and A/O-SBR. In the STH-SBR, the aerobic glycogen accumulation was lower than that in the A/O-SBR while the average idle phosphorus release was greater than that in the AEI-SBR. Finally, the key enzyme activities in the AEI and A/O regimes were compared for the first time, and the reasons for the AEI regime showing lower exopolyphosphatase and polyphosphate kinase activities were also discussed.