Abstract
In this paper, it has been experimentally proved for the first time that urate is present in human serum bound to proteins. The protein-bound urates, when liberated, absorb in the ultraviolet light at wavelengths shorter than 293 - 295 nm, the wavelength at which the pure uric acid solution gives his maximum peak [1] .
Highlights
In this paper, it has been experimentally proved for the first time that urate is present in human serum bound to proteins
According to the fact that uric acid has its maximum absorbance in the U.V. light between the W.Ls of 290 nm and 295 nm under optimized conditions and making use of the specific destructive effect of uricase on uric acid, the absorption spectrum of a serum sample was carefully studied in the region between 270–300 nm before and after incubation with uricase
The results showed a remarkable difference in the wave lengths (W.Ls) at which urate in serum gives its maximum absorbance, 283–290 nm, compared to 293 nm, the W.L. at which the pure uric acid solution gives its maximum band
Summary
It has been experimentally proved for the first time that urate is present in human serum bound to proteins. The protein-bound urates, when liberated, absorb in the ultraviolet light at wavelengths shorter than 293 - 295 nm, the wavelength at which the pure uric acid solution gives his maximum peak [1]. ; the presence of other protein-bound substances which after being free, give rise to an increase in the absorption spectrum in the region between 270 and 300 nm and not attacked by uricase has been established
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