A new composite fabricated from hydroxyapatite, gelatin-MgO microparticles, and compatibilized poly(butylene succinate) with osteogenic functionality

Abstract

In this study, thermally processed recycled fish teeth (FT) and fish scales, magnesium oxide (MgO), and biobased polyesters were fabricated into new bioactive and environmentally friendly composites. The magnesium oxide was encapsulated into laboratory-made fish scale-derived gelatin to form gelatin-MgO microparticles. Hydroxyapatite (HA) and gelatin were obtained by heat-treating FTs and fish scales, respectively. Compatibilized poly(butylene succinate) (CPBS), i.e., poly(butylene succinate) (PBS) to which had been added acrylic acid-grafted PBS (PBS-g-AA) compatibilizer, was combined with HA/gelatin-MgO (GHA) to form CPBS/GHA composites. The structure and tensile properties of the composites were investigated. The CPBS/GHA composites improved the adhesion and proliferation of osteoblast cells. Osteoblast growth, osteoclast growth inhibition, and the antibacterial effect of CPBS/GHA composites were primarily due to the slow release of magnesium ions into the environment from the gelatin-MgO microparticles. Higher levels of calcium and phosphorus species were observed for various PBS/HA and CPBS/GHA composites immersed in simulated body fluid. Mineralization measurements indicated that calcium and phosphate ions precipitated in osteoblasts placed on PBS/HA and CPBS/GHA composites. The study successfully developed a new composite material containing 5 wt% gelatin/MgO (phr), CPBS/HA 10 wt% and 1.0 % gelatin/MgO (an optimum formula of MgO). This composite exhibited superior tensile strength, antibacterial effect, osteoclast growth enhancement, and osteoclast growth reduction. These results suggest that the composites may facilitate the formation of new bone formation in vivo. The CPBS/GHA composites displayed good bone tissue repair ability in engineering applications.