Abstract

A simple and accurate method for the determination of erythrocyte reduced glutathione (GSH) is described. This method is based on the observation that when added to a pre-formed palladium-thiorpromazine complex which has an absorption maximum at 565 nm, GSH competitively displaces chlorpromazine from the complex, resulting in a decrease in absorbance which is in direct proportion to the quantity of GSH added. The procedure has been successfully used in the erythrocyte GSH stability test for screening glucose-6-phosphate dehydrogenase deficiency. With minor modifications in the analytical procedure, oxidised glutathione (GSSG) can also be assayed.

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