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Abstract
A method for the measurement of mean integrated temperature using a spectrophotometer to trace the course of a chemical reaction is described. The substrate, phenyl ,8-D glucopyranoside, has an acid catalysed hydrolysis reaction with a temperature response similar to that of biological processes. The accuracy of the technique (? 0.50C) is similar to the sucrose inversion method. Advantages of the new technique are 1) use of a spectrophotometer which is more commonly available than a polarimeter, 2) ability to measure in temperatures as low as -40'C and 3) storage of samples in an inactive state. Arrhenius parameters are given but it is recommended that these be calculated for each solution used. The temperature estimate is discussed in relation to environmental variability.
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