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Abstract
A new bioactive isoflavone glycoside with a molecular formula C<sub>34</sub>H<sub>42</sub>O<sub>20</sub>, melting point 261°C-263°C and [M]<sup>+</sup> 770 was isolated from the methanol soluble fraction of 90% methanolic extract of the stem of <em>Ficus arnottiana </em>Miq. The compound was illustrated as a new bioactive isoflavone glycoside 5,6,5ˈ-trihydroxy-8,3ˈ-dimethoxy-isoflavone 5ˈ-O-α-L-rhamnopyranosyl (1→3) O-α-L xylopyranosyl 5-O-β-D-glucopyranoside were characterized with the help of various chemical reactions, Fourier transforms infrared spectroscopy (FT-IR), Nuclear magnetic resonance spectroscopy (1HNMR), Mass spectrometry (LC-MS) and chemical degradations. Isolated compounds were also used for the antimicrobial activity of micro-organisms worked successfully tested against all strains: <em>Escherichia coli, Bacillus cereus, and Staphylococcus aureus, </em>while the least activity by<em> Pseudomonas aeruginosa</em>.
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