A new Bacillus Paralicheniformis sp. Tmas-01 as bioreactor for synthesis of Ag/AgCl composite-different effects of biological and Rodamin B dye decolorization, anticancer, genotoxic activity.

Abstract

The Ag/AgCl composite can be synthesized using various chemical, physical, and biological methods. Biosynthesis of nanoparticles is a rapid technology and has advantages over physical and chemical synthesis methods. This research is based on the bacterial formation of silver nanoparticles by bacterial strain Tmas-01. The bacterial strain Tmas-01 was isolated from soil samples and screened for its potential to form Ag/AgCl composite. The formation of Ag/AgCl composite was investigated at the 300-800nm range using UV-Vis spectrophotometer. Also, the structural and morphological synthesized nanoparticles were characterized using FTIR, SEM-EDS, and XRD. These results showed that Ag/AgCl composite have the SPR absorption peak at 410nm and a size range of 10-30nm with a spherical shape. The Ag/AgCl composite exhibited antibacterial and antibiofilm effects against uropathogenic organisms with a range of 5-100%. The results of the genotoxic effects of Ag/AgCl composite by Salmonella typhimurium reverse mutation bioassay (Ames test) indicated which Ag/AgCl composite had no mutation effect. Moreover, Ag/AgCl composite determined an anti-mutagenic effect with a percentage between 97 and 100%. Furthermore, the toxicity of biosynthesized nanoparticles was measured against brine shrimp to evaluate the cytotoxic effect and displayed LC50 (1µg/ml). The Ag/AgCl composite could effectively photocatalytic degrade up to 64% of Rhodamine B dye. The phylogenetic analysis showed that strain Tmas-01 belonged to Bacillus paralicheniformis sp. with 99.29% similarity.