A new and standardised method to sample and analyse vitreous biopsies

Abstract

AbstractPurpose To investigate and prepare a universal protocol for sampling and analysing vitreous material. Vitreous biopsies are difficult to handle because of the paucity of cells, the gelatinous formation and structure of the vitreous, the low frequency of biopsies and the burden of long‐during, delicate handwork by the technician.Methods After a standardised 23gauge vitrectomy, 50 consecutive vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automatic processor from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® ( contains a mucolyticum) and Preservcyt®.Results In 96% (48 of 50 cases) sufficient material was found for diagnosis. Cytolyt® wash was necessary in 15% of cases to prevent clotting of the delicate tubes in the Cellient®, this procedure causes a loss of cellular material. Immuno‐histovhemical stainings were equal in quality with both preservatives. Labour hours of processing by technician was diminished by 4, compared with former, not‐standardised techniques.Conclusion A standardised protocol for sampling and handling viteous biopsies by a 23G vitrectomy, fixing in PreservCyt® and processing by the Cellient® gives a superior result in morphology, number of cells, possibility of immuno‐histochemical stainings and technician labour hours.