Abstract
The effects of individual steps of industrial refining on the alteration of triacylglycerides (TAG) are reported. The level of dimer triglycerides, normally not present in crude oils, increased after each refining step, especially after steam-washing and desodorisation. A good correlation between the applied temperatures and dimer triglycerides content was found. The forming of dimer triglycerides starts at 90 °C and increases corresponding to the extension of thermal treatment like normal heating or desodorisation. The data for various types of vegetable oils demonstrate that there is no clear-cut different tendency to form dimers. Heated oils with different contents of linoleic acid produced nearly the same amount of dimers. Other criteria, like the determination of trans fatty acids, steradienes, or the UV-absorption, were found not to be appropriate to detect a thermal treatment at temperatures below 150—170 °C. The formation of steradienes mainly depends on the total sterol contents, the percentage of added bleaching earth, and its acidity and moisture. Over 160 commercial vegetable oil samples were analysed to obtain a data range on the content of dimerised triglycerides. Mostly, vegetable oils Iabelled as non-refined (which may be steam-washed) did not exceed dimer contents of 0.1%. Virgin vegetable oils did not contain dimers (< 0.05%). The content of dimer triglycerides in vegetable oils was determined by a new method via clean-up on a short silica gel column, followed by size-exclusion HPLC with refractive index detection.
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