Abstract

RATIONALE: Accurate diagnosis of mosquito allergy has been hampered by the labourious task of obtaining mosquito salivary allergens. We previously studied 3 recombinant Aedes aegypti salivary allergens (rAed a 1, rAed a 2, and rAed a 3), and wanted to express, purify and characterize another allergen, a 67 kDa alpha-glucosidase, Aed a 4.

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