A neural network underlying individual differences in emotion and aggression in male golden hamsters

Abstract

In rodents, aggressive behavior can be altered by experimental manipulations of emotional responsiveness. The goal of this study was to identify characteristics of emotional reactivity associated with individual differences in aggressive behavior and their integration within a common neural network. Male golden hamsters were first screened for offensive aggression. Then, the animals were trained through immediate reinforcement and tested for their adaptation to a delayed reward. Similar protocols have been used to test behaviors associated with frustration. At first, all hamsters showed increased frequency of bar pressing per reward during delayed reinforcement. However, Low-Aggression animals were able to adapt to the delay and showed a decreased rate of bar pressing per reward within 5 days. In contrast, High-Aggression animals maintained a high rate of bar pressing per reward. In addition, brains were collected after immediate reward training or delayed reward testing, and labeled for pCREB-immunoreactivity as a marker of trans-synaptic activity. In High-Aggression individuals, elevated density of cyclic AMP response element binding protein, phosphorylated (pCREB) immunostaining was found within the anterior hypothalamus, an area critical to the control of aggression. Delayed reinforcement was associated with enhanced pCREB immunostaining within the central amygdala, medial amygdala and preoptic area/hypothalamus continuum. Further analysis of the data also showed a positive correlation in labeling density between the lateral septum and the anterior hypothalamus, specifically in Low-Aggression animals exposed to delayed reward. Therefore, as High-Aggression individuals lack control of their emotional reactivity, they are also characterized by a de-synchronization between the inhibitory output of the septum and the aggression areas of the hypothalamus. Finally, our data also show that frustration is associated with an extensive activation of the preoptic area/hypothalamus continuum and amygdala.