A Network Pharmacology Technique to Investigate the Synergistic Mechanisms of Salvia miltiorrhiza and Radix puerariae in Treatment of Cardio-Cerebral Vascular Diseases.

Yang Ma,Yi Ding,Kai Wang,Lu Lei,Shaojie Huang,Wenjun Wang,Sha Zhang,Fei Li,Zhe Li,Aidong Wen,Jiani Yang

doi:10.1155/2020/6937186

Abstract

Objective This study is aimed to analyze the active ingredients, drug targets, and related pathways in the combination of Salvia miltiorrhiza (SM) and Radix puerariae (RP) in the treatment of cardio-cerebral vascular diseases (CCVDs). Method The ingredients and targets of SM and RP were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), and the disease targets were obtained from Therapeutic Target Database (TTD), National Center for Biotechnology Information (NCBI), and Online Mendelian Inheritance in Man (OMIM) Database. The synergistic mechanisms of the SM and RP were evaluated by gene ontology (GO) enrichment analyses and Kyoto encyclopedia of genes and genomes (KEGG) path enrichment analyses. Result A total of 61 active ingredients and 58 common targets were identified in this study. KEGG pathway enrichment analysis results showed that SM- and RP-regulated pathways were mainly inflammatory processes, immunosuppression, and cardiovascular systems. The component-target-pathway network indicated that SM and RP exert a synergistic mechanism for CCVDs through PTGS2 target in PI3k-Akt, TNF, and Jak-STAT signaling pathways. Conclusion In summary, this study clarified the synergistic mechanisms of SM and RP, which can provide a better understanding of effect in the treatment of CCVDs.

Highlights

Cardio-cerebral vascular diseases (CCVDs) seriously threaten human health and life for the high occurrence and mortality [1]
DL is mainly used to predict exactly how “drug like” an ingredient is, which helps to assist pharmacokinetic and pharmaceutical properties, for example, solubility and chemical stability [18]. e active ingredients of SM and RP were gathered from TCMSP [19], a phytochemical database, with the keywords “Salvia miltiorrhiza” and “Radix puerariae”. e oral bioavailability (OB) and DL of chemical ingredients of SM and RP were collected from TCMSP
In SM and RP, 61 active ingredients and 176 targets were obtained from TCMSP. e resulting compound-target network is shown in Figures 2(a)–2(c), respectively

Summary

Introduction

Cardio-cerebral vascular diseases (CCVDs) seriously threaten human health and life for the high occurrence and mortality [1]. Severe sequelae, complicated symptoms, and difficulties in treatment are markedly characteristics of CCVDs. CCVDs include cerebrovascular diseases and cardiovascular diseases, and about 40% of middle-aged and elderly people are suffering from hyperglycemia, hyperlipidemia, and hypertension [2]. Erefore, it is of great clinical significance to formulate effective treatment strategies for the significant characteristics of CCVD such as severe sequelae, complex symptoms, and difficult treatment [3]. E combination of SM and RP exerts synergistic effect on CCVDs. Studies have shown that SM and RP are well tolerated in the treatment of cardio-cerebral vascular diseases and can improve neointimal hyperplasia, vascular function, and structure and significantly improve the occurrence of atherosclerosis and hypertension [6, 7]

Methods

Results

Conclusion