A network analysis of miRNA mediated gene regulation of rice: crosstalk among biological processes.

Abstract

To understand the network architecture of miRNA mediated regulations at the genomic and functional levels of rice, we have made an unambiguous annotation of the experimentally verified miRNAs, predicted their targets and the possible biological functions they can affect. Some functions, namely translational and protein modifications and photosynthesis are targeted by higher percentage of miRNA. Using transformation procedures, we constructed a genome scale miRNA-miRNA functional synergistic network (MFSN). The analysis of MFSN modules help to identify miRNAs co-regulating target genes having several interrelated biological processes. Some of these target genes are also co-expressed under particular conditions. For example, the genes co-expressed under drought conditions as well as those targeted by miRNAs present in a MFSN module have interdependent biological processes namely, photosynthesis, cell-wall biogenesis, root development and xylan synthesis. The stress-induced miRNAs and their distributions, and the presence of transcription factors in the target set of MFSN modules were also analyzed.