Abstract
The nuclear pore complex (NPC) tethers chromatin to create an environment for gene regulation, but little is known about how this activity is regulated to avoid excessive tethering of the genome. Here we propose a negative regulatory loop within the NPC controlling the chromatin attachment state, in which Nup155 and Nup93 recruit Nup62 to suppress chromatin tethering by Nup155. Depletion of Nup62 severely disrupts chromatin distribution in the nuclei of female germlines and somatic cells, which can be reversed by codepleting Nup155. Thus, this universal regulatory system within the NPC is crucial to control large-scale chromatin organization in the nucleus.
Highlights
The nuclear pore complex (NPC) is the molecular hub for transport in and out of the nucleus
Ever since a physical association of the NPC with the genome was postulated (Blobel 1985), increasing evidence has pointed to a role for the NPC in tethering chromatin to create an environment for gene regulation
Meiotic chromatin becomes fully detached from the nuclear envelope and compacted into a spherical structure, the karyosome, after recombination in Drosophila oocytes (Fig. 1A; King 1970)
Summary
A negative loop within the nuclear pore complex controls global chromatin organization Manuel Breuer and Hiroyuki Ohkura. The nuclear pore complex (NPC) tethers chromatin to create an environment for gene regulation, but little is known about how this activity is regulated to avoid excessive tethering of the genome. Depletion of Nup severely disrupts chromatin distribution in the nuclei of female germlines and somatic cells, which can be reversed by codepleting Nup155. This universal regulatory system within the NPC is crucial to control large-scale chromatin organization in the nucleus. Specific gene regulation modules aside, little is known about how the association of chromatin at a global scale is regulated to avoid excessive, unwanted attachment and how misregulation affects chromatin organization.
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