Abstract
A new ratiometric fluorescent probe (XWJ) was developed on the basis of Förster resonance energy transfer (FRET). The probe constructed by dansyl and 2-(3,5,5-trimethylcyclohex-2-en-1-ylidene) malononitrile, exhibited a higher efficiency of energy transfer (up to 93.59%). The probe emerged excellent sensitivity and selectivity toward hypochlorous acid (HOCl). In particular, upon addition of HOCl, the probe can reveal a clear response in 25s and manifest an obvious change in fluorescence colour from red to bright yellow, which could be recognized by naked eyes. Furthermore, the calibration curve is linear in the concentration range of 0–55μM with the detection limit of 0.19μM, which means that the probe can detect HOCl in low concentration. Besides, the fluorescence emission of the acceptor arises near-infrared region (NIR) and the probe possesses a large pseudo Stokes shift (300nm) and well-separated dual emission (550/670nm). The probe showed outstanding sensitivity and selectivity toward HOCl in cell imaging.
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