A near-infrared Fluorescent Probe Based on Dicyanisophorone for the Detection of Zinc Ions (Zn2+) in Water and Living Cells.

Abstract

As one of the important metal ions, zinc ions (Zn2+) are widely involved in various physiological and pathological processes, and play fundamental roles in neurotransmission, cell metabolism and apoptosis. However, the convenient monitor of Zn2+ in environmental and biological samples remains challenging. In this study, a small molecule dicyanoisophorone-based schiff base incorporating with o-phenylenediamine was synthesized. It can rapidly combine with Zn2+ to emit significant near-infrared fluorescence (maximum emission wavelength: 660nm), so it can be used as a probe to quantitatively detect Zn2+ in the range of 0-10μM, with a detection limit as low as 4.8nM, showing the probe has high sensitivity for Zn2+. And the probe has a fast response time to Zn2+ (less than 30s) and a large Stoke-shift (179nm). In addition, the high recovery rates in practical water samples, and the clear fluorescent images in living A549 cells were obtained, which are of great significance for the detection of Zn2+ in the environment and biosystem. Due to its simple operation, good selectivity and anti-interference ability, short detection time and high sensitivity, this probe has great application potential as a fast detection tool for Zn2+ in environmental water and biological samples.