A near infrared fluorescence probe with dual-site for hydrogen sulfide and sulfur dioxide detection

Abstract

Both hydrogen sulfide (H2S) and sulfur dioxide (SO2) are regarded as double-edged swords. They are toxic gases at high concentration, and at low concentration they are beneficial to the human. Therefore, it is of great significance to develop single chemosensor which enable to detect them with different fluorescence signal changes. In this work, a novel dual-site fluorescence probe (AMN-SSPy) with near infrared emission (675 nm) was designed, which realized quantitative detection for H2S and SO2 by fluorescence enhancement and fluorescence quenching, respectively. AMN-SSPy showed advantages such as excellent selectivity to H2S and SO2, strong anti-interference ability, high sensitivity for H2S (LOD 1.03 µM for H2S and 77.08 µM for SO2) and low toxicity. In addition, AMN-SSPy possessed the capacity to successfully image the endogenous and exogenous H2S, and it was also used to demonstrate that Ca2+ could induce accumulation of H2S in cell and zebrafish. Finally, the rapid detection of SO2 by AMN-SSPy in real samples was also established.