A natural solution for Obesity  Devil´s Claw attenuates food intake via ghrelin receptor modulation

Abstract

Event Abstract Back to Event A natural solution for Obesity  Devil´s Claw attenuates food intake via ghrelin receptor modulation Cristina Torres-Fuentes1, Wessel F. Theeuwes1, Michael K. McMullen2, Anna K. McMullen3, John F. Cryan1, Timothy G. Dinan1 and Harriët Schellekens1* 1 University College Cork, Ireland 2 University of Westminster, United Kingdom 3 Life Force Research, Sweden Ghrelin is a stomach-derived peptide that acts as orexigenic hormone in the hypothalamus stimulating food intake through the growth hormone secretagogue receptor (GHS-R1a). Dysregulated ghrelin signalling may contribute to the development of metabolic disorders such as obesity. Current pharmacologic anti-obesity treatments lack efficacy and have shown severe side effects, highlighting the urgent need for novel strategies contributing to the maintenance of a healthy weight. Natural products are receiving special consideration as sources of bioactives with potential beneficial health effects which may be safer and more attractive for consumers than synthetic therapeutics. One example is the plant extract derived from the dried tuberous roots of Harpagophytum procumbens which has been used traditionally as herbal medicine for a variety of conditions, and currently is mainly used as an anti-inflammatory agent and as an analgesic. In addition, it has some folkloric precedent as a modulator of appetite. This study aims to investigate the effect of H. procumbens on GHS-R1a receptor modulation in vitro and analyse its effects on food intake in vivo. GHS-R1a receptor activating potential of Harpagophytum procumbens soluble extract was analysed by calcium mobilization and receptor internalization assays in human embryonic kidney cells (Hek) stably expressing the GHS-R1a receptor. Furthermore, cumulative food intake was investigated in male C57Bl/6 mice following intraperitoneal (IP) administration of the soluble H. procumbens extract. Exposure to H. procumbens extract demonstrated a significant increased cellular calcium influx but did not induce subsequent GHS-R1a receptor internalization, which is characteristic of full receptor activation. A significant anorexigenic effect was observed in male C57BL/6 mice following peripheral administration of H. procumbens extract. We conclude that H. procumbens root extract is a potential novel source of potent anti-obesity bioactives. These results reinforce the promising potential of natural bioactives to be developed into functional foods with weight-loss and weight maintenance benefits. Keywords: Obesity, ghrelin receptor, Appetite Regulation, Plants, Medicinal, Harpagophytum Conference: Neuroscience Ireland Young Neuroscientists Symposium 2014 , Dublin, Ireland, 20 Sep - 20 Sep, 2014. Presentation Type: Poster Presentation Topic: Early Career Neuroscience Citation: Torres-Fuentes C, Theeuwes WF, McMullen MK, McMullen AK, Cryan JF, Dinan TG and Schellekens H (2014). A natural solution for Obesity  Devil´s Claw attenuates food intake via ghrelin receptor modulation. Front. Neurosci. Conference Abstract: Neuroscience Ireland Young Neuroscientists Symposium 2014 . doi: 10.3389/conf.fnins.2014.87.00035 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Sep 2014; Published Online: 17 Sep 2014. * Correspondence: Dr. Harriët Schellekens, University College Cork, Cork, Ireland, s.chellekens@ucc.ie Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen John F Cryan Timothy G Dinan Harriët Schellekens Google Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen John F Cryan Timothy G Dinan Harriët Schellekens Google Scholar Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen John F Cryan Timothy G Dinan Harriët Schellekens PubMed Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen John F Cryan Timothy G Dinan Harriët Schellekens Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.