A Natural Recombinant between the Geminiviruses Tomato yellow leaf curl Sardinia virus and Tomato yellow leaf curl virus Exhibits a Novel Pathogenic Phenotype and Is Becoming Prevalent in Spanish Populations

Abstract

This work provides evidence of the significant contribution of recombination to the genetic diversification of emerging begomovirus populations. In southern Spain, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV) are distinct geminivirus species that coexist in the field and contribute to the tomato yellow leaf curl disease epidemic. A natural recombinant between TYLCSV and TYLCV has been detected and an infectious clone of a recombinant isolate (ES421/99) was obtained and characterized. Analysis of its genome showed that the recombination sites are located in the intergenic region in which a conserved stem-loop structure occurs and at the 3′-end of the replication enhancer protein open reading frame. ES421/99 exhibited a novel pathogenic phenotype that might provide it with a selective advantage over the parental genotypes. This agrees with results from field studies which revealed that the recombinant strain is becoming prevalent in the region in which it was detected.