A natural pancreatic 3D bioscaffold platform for in vitro study on exocrine pancreas

Abstract

Objective To evaluate the biochemical function of rat nature three-dimensional acellular pancreatic bioscaffold (3D-APB) in promoting cell proliferation and differentiation in vitro. Methods The fresh pancreas from 10 rats were perfused to prepare 3D-APB. The biocompatibility of 3D-APB was eva-luated. The experiment was divided into 4 groups based on 4 kinds of three-dimensional media for AR42J culture, including blank control group, ECM group, PLGA group and 3D-APB group. We compared the proliferation and differentiation of AR42J pancreatic acinar cell at 3 days, 5 days, 7 days and 10 days after seeding among 4 groups. Results The 3D-APB could represent a biocompatible scaffold capable of integrating within host tissue. The proliferation rate of AR42J cells by MTT in 3D-APB was higher, while the apoptosis rate by flow cytometry was lower than those in other 3 media, which were all significantly different (P<0.05), respectively. The protein expression of PDX-1 and PTF-1 by western blot in 3D-APB group was greatly higher than those in other 3 groups (both P<0.05). The mRNA expression of PDX-1 and PTF-1 through qRT-PCR was significantly higher than those in other 3 groups (both P<0.05). Conclusion Compared with the commonly used chemical and natural scaffold at present, 3D-APB could promote cell proliferation and differentiation, which was more appropriate for regenerative medicine. Key words: Acellular scaffold; Acellular matrix; Pancreas; Acinar cell; Proliferation; Differentiation