Abstract
A sensitive and selective electrochemical aptasensor based on a novel composite film consisting of carbon black (CB) and graphene oxide@Fe3O4was developed for detection of chlorpyrifos. Chitosan (CS) functionalized CB has high specific surface area, ideal dispersibility and good electrical conductivity, thus, we used them to capture more graphene oxide@Fe3O4 and further amplify the current signal of the aptasensor. The graphene oxide@Fe3O4 nanocomposite formed a unique sensing film with strong synergistic effects, the graphene oxide provided a large surface area, whereas Fe3O4 was uniformly deposited on the graphene oxide, facilitating electron transfer for sensitive detection of chlorpyrifos with excellent selectivity and a low detection limit. Immobilization of the probe Apt on the electrode was greatly improved owing to the unique synergistic effects of graphene oxide@Fe3O4. Therefore, the integration of graphene oxide@Fe3O4 nanocomposite with biomolecules chlorpyrifos aptamer (Apt) as a biorecognition element constructed a novel biosensing platform. Under the optimized conditions, the proposed aptasensor provided a linear range of 0.1–105ng/mL with a low detection limit of 0.033ng/mL. The aptasensor performed good selectivity, reproducibility and stability. Additionally, the proposed aptasensor can be applied to monitor chlorpyrifos residues in real vegetable samples.
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